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  • 3562 次 Isolation and cultivation of endothelial progenitor cells (EPCs) 2011-9-30
    Isolation and cultivation of endothelial progenitor cells (EPCs) Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue rege

  • 1996 次 Isolation of dermal mast cell (DMC) 2011-9-22
    Isolation of dermal mast cell (DMC) 1.Human DMC were isolated from skin by enzymatic digestion.2.Dermal tissue was obtained from patients with skin after informed consent was give

  • 9417 次 Isolation of retinal pigment epithelial cell 2011-9-16
    Isolation of retinal pigment epithelial cell The pigmented layer of retina or retinal pigment epithelium (RPE) is the pigmented cell layer just outside the neurosensory retina that

  • 2673 次 Trabecular cell monolayer culture 2011-9-2
    Trabecular cell monolayer cultureOriginally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves

  • 2469 次 Isolation of rodent pancreatic β cells 2011-8-1
    Isolation of rodent pancreatic β cells 1.Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2.Rat islets were isolated from mal

  • 2553 次 Culture of human renal proximal tubule cells and renal cortical fibroblasts 2011-7-11
    Culture of human renal proximal tubule cells and renal cortical fibroblasts 1.The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibrobla

  • 2292 次 Isolation of human colonic epithelial cells (EC) 2011-6-21
    Isolation of human colonic epithelial cells (EC)1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were

  • 2474 次 Primary brain cell isolation and culture 2011-6-13
    Primary brain cell isolation and culture. 1.Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containin

  • 4126 次 xCELLigence系統(tǒng)實時監(jiān)測低毒性X-tremeGENE DNA轉(zhuǎn)染實驗 2011-6-10
    xCELLigence系統(tǒng)實時監(jiān)測低毒性X-tremeGENE DNA轉(zhuǎn)染實驗前言X-tremeGENE DNA Transfection Reagent是一種非脂質(zhì)體、多組分轉(zhuǎn)染試劑，已被證明可有效轉(zhuǎn)染多種細胞。X-tremeGENE 9和X-tremeGENE H

  • 3325 次 滲透脅迫后擬南芥根表皮細胞膨壓恢復(fù)中離子吸收的作用 2011-5-6
    滲透脅迫后擬南芥根表皮細胞膨壓恢復(fù)中離子吸收的作用 注：滲透脅迫誘導(dǎo)的細胞膨壓和K+離子流的動力學(xué)變化。高滲處理導(dǎo)致膨壓快速下降，同時K+內(nèi)流增加，膨壓在40min時恢復(fù)，K+內(nèi)流減小。 提高

  • 2459 次 Measurement of primary endothelial cell permeability to fluxes of dextran or albumin 2011-4-1
    Measurement of primary endothelial cell permeability to fluxes of dextran or albuminThe fluxes of albumin or dextran across vascular endothelial cell (ECs) were evaluated using Tra

  • 15342 次 Isolation and Culture of Human Brain Tumor Stem Cell 2011-4-1
    Isolation and Culture of Human Brain Tumor Stem CellThe isolation, culture, identification, and purification of stem cells from primary human brain tumors of different phenotypes h

  • 2569 次 Identification and expansion of the tumorigenic lung cancer stem cell population 2011-4-1
    Identification and expansion of the tumorigenic lung cancer stem cell populationLung cancer contains a rare population of CD133+ cancer stem-like cells able to self-renew and gener

  • 2577 次 Isolation and Culture of Human Liver Stem Cells 2011-4-1
    Isolation and Culture of Human Liver Stem CellsMaterials and Methods1.Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy li

  • 2335 次 FITC標(biāo)記抗體-Marsshall氏法 2010-10-13
    FITC標(biāo)記抗體-Marsshall氏法材料：抗體球蛋白溶液、0.5mol/L pH9.0碳酸鹽緩沖液、無菌生理鹽水、異硫氰酸熒光素、1%硫柳汞水溶液、三角燒瓶（25—50ml）、冰及冰槽（或1000ml燒杯）、電磁攪拌器

  • 4210 次 xCELLigence 系統(tǒng)內(nèi)源性GPCRs 細胞功能研究 2010-10-11
    xCELLigence系統(tǒng)實時評測內(nèi)源性G-偶聯(lián)蛋白受體（GPCRs）功能Jeff Irelan 美國圣地亞哥ACEA Biosciences 公司Jonathan H. Morgan 美國弗吉尼亞州Manassas ATCC 產(chǎn)品經(jīng)理前言 G-偶聯(lián)蛋白受體（GPC

  • 3163 次 原代細胞骨架的染色方法 2010-10-11
    原代細胞骨架的染色方法微絲的顯示方法步驟：1. 用PBS液漂洗蓋片培養(yǎng)的原代細胞3次，每次30s；2. 用2%的甲醛/PBS液固定原代細胞3min；3. 用0.5%的三硝基甲苯/PBS處理3次，每次10min；4. PBS漂洗

  • 3101 次 原代細胞富爾根（Feulgen）反應(yīng)顯示DNA 2010-10-11
    原代細胞富爾根（Feulgen）反應(yīng)顯示DNA基本原理：顯示DNA的傳統(tǒng)方法是富爾根（Feulgen）反應(yīng)，切片先用稀鹽酸處理，DNA經(jīng)弱酸（1mol/L HCL）水解后，在60℃條件下使DNA分子中脫氧核糖與嘌呤之間

  • 6694 次 xCELLigence系統(tǒng)實時檢測神經(jīng)毒性 2010-10-11
    xCELLigence系統(tǒng)持續(xù)檢測神經(jīng)細胞培養(yǎng)Sebastian Diemert, Julia Grohm, Svenja,Tobaben, Amalia Dolga, Carsten Culmsee德國，馬爾堡大學(xué)，藥理學(xué)與臨床藥學(xué)研究所 摘要：為了研究類神經(jīng)元細胞H

  • 2793 次 原代細胞DNA與RNA的吖啶橙熒光染色法 2010-10-9
    原代細胞DNA與RNA的吖啶橙熒光染色法基本原理：吖啶橙（acridine orange，AO）是一種常用熒光染料，吖啶橙與原代細胞內(nèi)的DNA、RNA都有親和力，但有一定的特異性，即結(jié)合后發(fā)不同顏色的熒光，DNA

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